Fig. 3.

Tau_294–305 epitope to hepatitis B core immunodominant region (T294-HBc) vaccination improved cognitive capacity in Tau.P301S mice. a Schematic diagram of behavioral test battery. b In the forced Y-maze, the time that mice spent in the new arm was recorded in 30-minute intertrial intervals. One-way analysis of variance (ANOVA) with LSD was used for statistical analysis. c The number of mice entries to the new arm. Statistics were analyzed by Mann-Whitney U test. d The learning and memory of Tau.P301S mice tested by novel object recognition (NOR). NOR discrimination index was determined by calculating (Time_novel − Time_old)/(Time_novel + Time_old), where 0 equals no preference for either object. One-way ANOVA with LSD was used for statistical analysis. e Latency to reach the hidden platform in the acquisition period of the Morris water maze (MWM). For 5 consecutive days, each mouse was subjected to two 60-second trials per day to find the hidden platform. The recorded data were analyzed and showed the changes of escape latency to find the hidden platform over the 5 days of training. Statistical significance was analyzed by two-way ANOVA. f Latency to reach the hidden platform location in the probe trial of the MWM without the platform. One-way ANOVA with LSD was used for statistical analysis. g The number of times of crossing the platform location during the memory trial in the MWM probe test without the platform. Statistics were analyzed by Mann-Whitney U test. h The time spent in the target quadrant in the MWM probe test. One-way ANOVA with LSD was used for statistical analysis. i The short-term memory of Tau.P301S mice. Spontaneous Y-maze tests were conducted to assess short-term memory of Tau.P301S mice treated with or without T294-HBc virus-like particles. The percentage of alternation was calculated as (actual alternations/maximum alternations) × 100%. One-way ANOVA with LSD (compared with adjuvant- and HBc-treated mice; *P < 0.05) was used for statistical analysis