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. 2018 Apr 21;17:213–223. doi: 10.1016/j.redox.2018.04.018

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig S1 — Effect of irradiation on GSH biosynthesis in macrophages from PTP1B WT and PTP1B KO mice. (A) Cells were exposed to 5 Gy and the mRNA levels of the catalytic and regulatory subunits of γ-glutamyl-cysteine ligase (Gclc and Gclm) and glutathione synthetase (Gss) were determined at 24 h. (B) The activity of glutathione synthetase (GS) was determined in cell extracts. Results show the mean±SD of three experiments. *P < 0.05 vs. the same condition in the WT cells; ^aP < 0.05; vs. the 0 Gy condition. Fold induction (F.I., A).