Table 2.

Differentially expressed (DE) genes identified in Mycoplasma suis -infected pigs tested by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR)

	Gene ID	Gene symbol	Description	qRT-PCR FC	RNA-Seq FCa	qRT-PCR p-value	RNA-Seq p-value**
DE up-regulated genes	100145895	ISG15	ISG15 ubiquitin-like modifier	2.79	4.72	0.07	0.04
DE down-regulated genes	100513324	IL22RA2	Interleukin 22 receptor, alpha 2	0.01	0.02	0.03	0.01
	574058	CD274	CD274 molecule	0.11	0.17	0.01	0.01
	100514901	BCL2L14	BCL2-like 14 (apoptosis facilitator)	0.24	0.25	0.01	0.07
	414371	CCR5	chemokine (C–C motif) receptor 5	0.37	0.33	0.04	0.03
	397644	CD180	CD180 molecule	0.37	0.26	0.01	0.02
	397271	AIF1	Allograft inflammatory factor 1	0.19	0.20	0.00	0.01
	397683	IL15	Interleukin 15	0.22	0.24	0.03	0.02
	100524563	PTPRO	Protein tyrosine phosphatase, receptor type, O	0.40	0.28	0.01	0.01
	397384	TLR8	Toll-like receptor 8	0.40	0.32	0.04	0.05

FC: fold change of a DE gene in the infected group related to the control group, GAPDH was used as reference control gene for calculation of relative quantification.

** p-values based on Cufflinks results. Note that a FC of 0.01 of a down-regulated gene indicates that infected group has 1/100 the amount of target RNA as the control group.

^aFC based on Cufflinks results.