Fig. 1.

GOLPH3 was overexpressed in tissue samples and HCC cell lines; a Expression of GOLPH3 mRNA in 60 pairs of paraffin-embedded HCC and adjacent normal liver samples were examined by qPCR. b Expression of GOLPH3 protein in 60 pairs of paraffin-embedded HCC and adjacent normal liver samples were examined by immunohistochemistry. The Magnification of immunohistochemistry images was 400×. c Expression of GOLPH3 mRNA of hepatocellular carcinoma cell lines (Bel-7402, QGY-7703, MHCC97L and MHCC97H) was examined by qPCR. d Expression of GOLPH3 protein of hepatocellular carcinoma cell lines (Bel-7402, QGY-7703, MHCC97L and MHCC97H) was examined western blotting. Control was a piercing liver tissue which was diagnose as hepatitis without tumor. The expression levels were normalized to GAPDH. Data was presented as the mean ± SD. P-values were calculated using Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001