Table 3.

Comparison of run times and memory usage of pipeline for models of different sizes

Model	Reactions	Peak	Parameter	Parameter	Model	Total
	(metabolites)	memory	search	balancing	building
		(GB)	(min:sec)	(min:sec)	(min:sec)	(min:sec)
E. coli	2583	29.6	22:05	33:14	2:12	57:33
iJO1366	(1805)
E. coli	95	0.2	0:58	0:04	0:07	1:10
Core model	(92)
S. cerevisiae	1612	19.1	13:18	11:44	1:14	26:18
iTO977	(2245)
L. lactis	21	0.2	0:34	0:01	0:01	0:41
	(26)

All values were calculated using 12 x 2.1 GHz cores of an AMD Opteron Processor 6272

Peak memory = maximum amount of RAM used

Databases were searched locally offline so that online connection speeds did not influence results