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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: Cell Host Microbe. 2018 Jun 13;23(6):832–844.e6. doi: 10.1016/j.chom.2018.05.002

Figure 2. Antigenic profile and CD4 binding of interdomain-locked Env trimers.

Figure 2

(A) Reactivity of unmutated and interdomain-stabilized BG505 SOSIP.664 trimers with a panel of anti-Env mAbs and sCD4 in ELISA. Mean optical density (OD) values at 450 nm for each mAb (complexed or not with sCD4) and for two different forms of sCD4 (sCD4-4d and CD4-Ig) are shown from a representative experiment performed in duplicate wells. The colors denote increased (blue) or decreased (red) binding to each stabilized trimer compared to the unmutated BG505 SOSIP.664 trimer.

(B) Dose-response reactivity of purified BG505 SOSIP.664 trimer and two interdomain-stabilized variants with a panel of anti-Env antibodies and 4-domain soluble CD4 (sCD4-4d) in ELISA. The data shown are mean ± SEM.

(C) Surface plasmon resonance (SPR) analysis of sCD4 binding to unmutated and interdomain-stabilized BG505 SOSIP.664 trimers. See also Figure S5 and Table S3.