Skip to main content
. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: Cell Host Microbe. 2018 Jun 13;23(6):832–844.e6. doi: 10.1016/j.chom.2018.05.002

Figure 4. The interdomain-locking design can be successfully applied to membrane-expressed, native full-length gp160.

Figure 4

(A) Flow cytometry analysis of binding of a panel of anti-Env mAbs and sCD4 to cell-surface-expressed WT and interdomain-stabilized full-length HIV-1 Env (gp160) of different clades: BG505 (clade A), BaL and JR-FL (clade B) and 1086c (clade E). The HIV-1 gp160 were expressed on the surface of 293T cells and tested at 48-72 hours post-transfection. The results show normalized mean fluorescence intensity (MFI) values from a representative experiment, relative to the MFI of mAb 2G12 (VRC01 for Env 1086c, which is not reactive with 2G12).

(B) Infectivity of HIV-1 pseudoviruses bearing WT or mutated BG505 Env in TZM-bl cells. Hill slope nonlinear regression curves are shown for samples with detectable infectivity.