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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: Cell Host Microbe. 2018 Jun 13;23(6):832–844.e6. doi: 10.1016/j.chom.2018.05.002

Figure 5. CD4-induced conformational changes disrupt the native-like antigenic state of unmutated, but not of interdomain-locked HIV-1 Env trimers.

Figure 5

(A) Effect of the CD4-mimetic miniprotein M48U1 on the antigenicity of unmutated and interdomain-locked SOSIP.664 trimers from two HIV-1 strains (BG505, clade A, and JR-FL, clade B), as assessed by ELISA.

(B) Effect of soluble CD4 (sCD4) on the antigenicity of unmutated and interdomain-locked SOSIP.664 trimers from BG505 and JR-FL, as assessed by ELISA.

The results show mean optical density (OD) values at 450 nm for each mAb in the presence or absence of M48U1 or sCD4 from a representative experiment performed in duplicate wells.