REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal anti-HIV-1 Gp120 2G12	NIH AIDS Reagent Program	Cat# 1476
Monoclonal anti-HIV-1 Gp120 b12	NIH AIDS Reagent Program	Cat# 2640
Monoclonal anti-HIV-1 Gp120 VRC01	NIH AIDS Reagent Program	Cat# 12033; RRID: AB_2491019
Monoclonal anti-HIV-1 Gp120 F105	NIH AIDS Reagent Program;	Cat# 857
Monoclonal anti-HIV-1 Gp120 PGT121	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 PG9	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 PG16	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 PGT145	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 CH01	NIH AIDS Reagent Program	Cat# 12561; RRID: AB_2491055
Monoclonal anti-HIV-1 Gp120 CAP256.09 (VRC26.03)	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 PGT151	John R. Mascola, VRC, NIH	N/A
Monoclonal anti-HIV-1 Gp120 35O22	NIH AIDS Reagent Program	Cat# 12586
Monoclonal anti-HIV-1 Gp120 8ANC195	Michel C. Nussenzweig, Rockefeller University	RRID: AB_2491037
Monoclonal anti-HIV-1 Gp120 447-52D	NIH AIDS Reagent Program	Cat# 4030
Monoclonal anti-HIV-1 Gp120 19b	NIH AIDS Reagent Program	Cat# 11436
Monoclonal anti-HIV-1 Gp120 48d	NIH AIDS Reagent Program	Cat# 1756
Monoclonal anti-HIV-1 Gp120 412d	John R. Mascola, VRC, NIH	N/A
Goat anti-human IgG-PE	SouthernBiotech	Cat# 2040-09
HRP-conjugated anti-human IgG, Fcr fragment Ab	Jackson ImmunoResearch	Cat# 109-035-008
Peroxidase AffiniPure Donkey Anti-Rabbit IgG	Jackson ImmunoResearch	Cat# 711-035-152
Bacterial and Virus Strains
NEB 5 – alpha Competent E. coli	New England BioLabs	Cat# C2987H
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Lectin from Galanthus nivalis	SIGMA-ALDRICH	Cat# L8275-5MG
BG505.T332N.SOSIP.664 gp140 trimer	This study	N/A
BG505.T332N.SOSIP.664.113C.429GCG gp140 trimer	This study	N/A
BG505.T332N.SOSIP.664.113C.431GCG gp140 trimer	This study	N/A
JR-FL.SOSIP.664 gp140 trimer	This study	N/A
JR-FL.SOSIP.664.113C.432GCG gp140 trimer	This study	N/A
DU422.SOSIP.664 gp140 trimer	This study	N/A
DU422.SOSIP.664.113C.432GCG gp140 trimer	This study	N/A
M48U1	Loic Martin, CEA (Martin et., 2003)	N/A
Recombinant Human sCD4 Protein	R&D Systems	Cat# 514-CD
Recombinant CD4-Ig2	NIH AIDS Reagent Program	Cat# 11780
Critical Commercial Assays
Luciferase 1000 Assay System	Promega	Cat# E4550
QuickChange II Site-Directed Mutagenesis Kit	Agilent	Cat# 200524
Deposited Data
BG505.113C-429GCG bound to PGT122 Fab and 35O22 Fab	This study	PDB: 6DE7; Deposition ID: D_1000233685
Experimental Models: Cell Lines
Human: HEK 293T, female	ATCC	Cat# CRL-3216
Human: HeLa-derived TZM-bl, female	NIH AIDS Reagent Program	Cat# 8129-442
Human: HEK 293FS (FreeStyle), female	Thermo Fisher	Cat# R79007
Experimental Models: Organisms/Strains
New Zealand white rabbits, female	Purchased from USDA-licensed breeders by Pocono Farm & Laborator y	Animals were identified by implanted ear clips
Oligonucleotides
Recombinant DNA
Plasmids pPPI4-BG505.T332N.SOSIP.664	John P. Moore, Weill-Cornell Medical College (Sanders et al., 2013)	N/A
Plasmids pPPI4-DU422.K295N.D386N.SOSIP.664	John P. Moore, Weill-Cornell Medical College (Julien et al., 2015)	N/A
Plasmids pVRC8400-JR-FL.SOSIP.664	Peter Kwong, NIH (Stewart-Jones et al., 2016)	N/A
Plasmids HIV-1 BG505 env expression vector	NIH AIDS Reagent Program	Cat# 11518
Plasmids pCAGGS HIV-1 JR-FL gp160 expression plasmids	James Binley, San Diego Biomedical Research Institute (Binley et al., 2003)	N/A
Plasmids pCDNA3.1 HIV-1 clone BaL.26	NIH AIDS Reagent Program	Cat# 11518
Plasmids pSG3 ∆env	NIH AIDS Reagent Program	Cat# 11051
Plasmids Panel of Global HIV 1 Env Clones	NIH AIDS Reagent Program	Cat# 12670
Software and Algorithms
FlowJo v.9.9.4	FlowJo, LLC	https://www.www.flowjo.com; RRID: SCR_008520
IMGT/V-QUEST	International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule.lefranc@igh.cnrs.fr), University of Montpellier, France	www.imgt.org; RRID: SCR_012780
Pymol	The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC.	https://pymol.org/2/
Cloanalyst	Thomas B. Kepler (tbkepler@bu.edu), Boston University	http://www.bu.edu/computationalimmunology/research/software/
HKL2000 Suite 6	HKL Research	www.hkl-xray.com
Phenix	Paul D. Adams (pdadams@lbl.gov), Lawrence Berkeley Laboratory	www.phenix-online.org; RRID: SCR_014224
Crystallographic Object-Oriented Toolkit (COOT) Software	Paul Emsley (paul.emsley@mrc-lmb.cam.ac.uk), Oxford University	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/; RRID: SCR_014222
WebLogo	Steven Brenner, Berkeley (Crooks et al., 2004)	http://weblogo.berkeley.edu
PRISM 7	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
TM Adjuvant: Adjuplex	Sigma-Aldrich	A0362