Fig. 1.

SH-SY5Y neuronal differentiation procedure and CORM-A1 improves neuronal differentiation yield. (A) SH-SY5Y cells were induced to differentiate during 7 days (d7) with 10 μM of RA treatment with or without 25 μM of CORM-A1, subjected to a differentiation medium exchange at day 4. After 7 days of differentiation (d7), a mixed population of undifferentiated cells and post-mitotic neurons was obtained. In order to obtain an enriched neuronal population, cultures are treated with anti-mitotic compounds for 5 days (d12). (B, C) Characterization of SH-SY5Y cells by immunocytochemistry (green staining: Tuj1; blue staining: DAPI; red staining: ki67; magnification 100 ×); (D) nuclei counting of total mixed cell population, using RA at 10 μM as reference and (E) nuclei count of neuronal enriched cell population. After 7 days of differentiation (B, D) and after anti-mitotic treatment during 5 days (C, E). The used statistical analysis was unpaired two-tailored t-test.