Fig. 3.

CO modulation of pentose phosphate pathway. (A) GSH/GSSG ratios were obtained 7 days after CORM-A1 supplementation; unpaired t-test with * p < 0.05; (B) mRNA expression of specific PPP enzymes (PGDH for phosphogluconate dehydrogenase and TKT for transketolase) in SH-SY5Y mixed cell population after 7 days of neuronal differentiation. RPL22 was used as house keeping gene and mRNA expression in RA-treated cells was used as reference, with ** p < 0.01 and # p < 0.1; (C) Protein expression of G6PD after 24 h and 7 days of neuronal differentiation assessed by western blot, with # p < 0.1; (D) Quantification of enzymatic activity (mU/mg of protein) of G6PD in the presence of RA or RA+CORM-A1 after 7 days of neuronal differentiation. The used statistical analysis was paired two-tailored t-test for panels A, B and C, while for panel D it was used unpaired t-test.