Table 2.
Changes in expression of oxidative stress response and antioxidant defence genes in HT29 cells after treatment with exogenous GSH and catechins at concentrations of 1 and 10 μM for 24 h at 37 °C.
| Antioxidant | Concentration [μM] | Gene | Description | Fold change | p-value |
|---|---|---|---|---|---|
| Up-regulation | |||||
| GSH | 1 | GSTZ1 | Glutathione transferase zeta 1 | 2.242 | 0.006 |
| NOX5 | NADPH oxidase 5 | 5.114 | 0.016 | ||
| SEPP1 | Selenoprotein P, plasma, 1 | 3.948 | 0.004 | ||
| TXNRD2 | Thioredoxin reductase 2 | 2.126 | 0.009 | ||
| 10 | BNIP3 | BCL2/adenovirus E1B | 3.813 | 0.030 | |
| NCF1 | Neutrophil cytosolic factor 1 | 5.570 | 0.001 | ||
| NOS2 | Nitric oxide synthase 2 | 2.792 | 0.013 | ||
| NOX5 | NADPH oxidase 5 | 3.625 | 0.019 | ||
| SEPP1 | Selenoprotein P, plasma, 1 | 2.831 | 0.012 | ||
| C | 1 | ALB | Albumin | 3.303 | 0.002 |
| CCL5 | Chemokine (C-C motif) ligand 5 | 3.492 | 0.021 | ||
| HSPA1A | Heat shock 70-kDa protein 1A | 2.629 | 0.040 | ||
| 10 | – | – | – | – | |
| EC | 1 | ALB | Albumin | 2.001 | 0.023 |
| 10 | HSPA1A | Heat shock 70-kDa protein 1A | 2.189 | 0.019 | |
| EGC | 1 | ALB | Albumin | 3.049 | 0.035 |
| CCL5 | Chemokine (C-C motif) ligand 5 | 2.767 | 0.020 | ||
| 10 | – | – | – | – | |
| ECG | 1 | AOX1 | Aldehyde oxidase 1 | 2.103 | 0.034 |
| HSPA1A | Heat shock 70-kDa protein 1A | 3.751 | 0.013 | ||
| 10 | – | – | – | – | |
| EGCG | 1 | ALB | Albumin | 2.836 | 0.014 |
| HSPA1A | Heat shock 70-kDa protein 1A | 3.351 | 0.005 | ||
| 10 | – | – | – | – | |
| Down-regulation | |||||
| GSH | 1 | CYGB | Cytoglobin | − 2.506 | 0.008 |
| 10 | – | – | – | – | |
| C, EC, ECG, EGCG | 1, 10 | – | – | – | – |
| EGC | 1 | – | – | – | – |
| 10 | SRXN1 | Sulfiredoxin 1 | − 2.542 | 0.012 | |