Table 4.
Characteristics of the gene cluster 31 of Streptomyces sp. Go-475 presumably involved in the biosynthesis of 8-O-Methyltetrangomycin.
| Gene | Putative product | Putative function |
|---|---|---|
| orf1 | TetR family transcriptional regulator | Efflux regulation |
| orf2 | MFS family transporter | Compounds' efflux |
| orf3 | Response regulator, LuxR family | Regulation of biosynthesis |
| orf4 | Acetyl-CoA carboxylase | Biosynthesis of malonyl-CoA |
| orf5 | Polyketide cyclase | Cyclization of the polyketide chain |
| orf6 | Beta-ketoacyl synthase | Polyketide biosynthesis |
| orf7 | Beta-ketoacyl synthase CLF | Polyketide chain length factor |
| orf8 | Acyl carrier protein | Polyketide biosynthesis |
| orf9 | 3-oxoacyl-ACP reductase | 1st reductive step in angucycline biosynthesis |
| orf10 | Polyketide cyclase | 1st cyclization/dehydration |
| orf11 | acyl-CoA carboxylase subunit beta | Biosynthesis of malonyl-CoA |
| orf12 | acyl-CoA carboxylase subunit epsilon | Biosynthesis of malonyl-CoA |
| orf13 | Monooxygenase FAD-binding | Oxygenation at C12? |
| orf14 | Monooxygenase FAD-binding | Oxygenation at C12? |
| orf15 | SDR oxidoreductase | unknown |
| orf16 | MFS family transporter | Compounds' efflux |
| orf17 | Antibiotic biosynthesis monooxygenase | unknown |
| orf18 | O-methyltransferase | Conversion of Tetrangomycin to 8-O-methyltatrangomycin |
| orf19 | Antibiotic biosynthesis monooxygenase | unknown |
| orf20 | SDR oxidoreductase | unknown |
| orf21 | Antibiotic biosynthesis monooxygenase | unknown |
| orf22 | S-adenosylmethionine synthetase | Provision of a methyl donor for O-methyltransferase (ORF18) |