Fig. 1.

CP and HEPH protein and mRNA expression in the pancreas. (A) Expression of CP and HEPH protein in lysates from the pancreas of WT, Heph/Cp KO, Cp KO, and Heph KO mice (n = 4 per group). Tubulin was used as a loading control. The western blot signals were quantified, and values were normalized to tubulin expression and expressed as a proportion of the WT control. (B) Quantitative real-time PCR analysis of Cp and Heph mRNA expression in the pancreas of WT, Heph/Cp KO, Cp KO, and Heph KO mice (n = 6 per group). Values were normalized to GAPDH expression and expressed as a proportion of the WT control value. Data are shown as mean ± SEM. Bars without common letters are significantly different (p < 0.05, as determined by one way ANOVA followed by Tukey's multiple comparisons test).