Fig. 2.

Heph/Cp KO mice exhibit severe iron overload in the pancreas. (A) Expression of ferritin protein in lysates from the pancreas of WT, Heph/Cp KO, Cp KO, and Heph KO mice (n = 4 per group). Tubulin was used as a loading control. The western blot signals were quantified, and values were normalized to tubulin expression and expressed as a proportion of the WT control value. (B) Non-heme iron concentration of the pancreas of WT, Heph/Cp KO, Cp KO, and Heph KO mice (n = 6 per group). (C) Macroscopic appearance of the pancreas of WT, Heph/Cp KO, Cp KO, and Heph KO mice. Data are shown as mean ± SEM. Bars without common letters are significantly different (p < 0.05, as determined by one way ANOVA followed by Tukey's multiple comparisons test) (For interpretation of the references to color in this figure, the reader is referred to the web version of this article.).