Table 1. Summary of the obtained read counts from long-read sequencing aligned to the PRV genome.
Samplea | Number of PRV readsb | Number of mapped readsc |
---|---|---|
MinION-Cap-selected | 120394 | 131223 |
MinION-cDNA | 44006 | 47363 |
MinION-RNA | 29832 | 30667 |
Sequel | 12555 | 13481 |
RSII-PolyA-amplified-1h | 6956 | 6956 |
RSII-PolyA-amplified-4h | 22958 | 22958 |
RSII-PolyA-amplified-8h | 46147 | 46147 |
RSII-PolyA-amplified-1, 2, 4, 6, 8h-Bluepippin 0.8kb-5kb+ | 1077 | 1077 |
RSII-PolyA-amplified-1, 2, 4, 6, 8h-Bluepippin 0.8-2kb | 7728 | 7728 |
RSII-PolyA-amplified-1, 2, 4, 6, 8h-Bluepippin 2-3kb | 6131 | 6131 |
RSII-PolyA-amplified-1, 2, 4, 6, 8h-Bluepippin 3-5kb | 3705 | 3705 |
RSII-PolyA-amplified-1, 2, 4, 6, 8h-Bluepippin 5kb+ | 4061 | 4061 |
RSII-PolyA-amplified-1, 4, 8h,-Manual Gel 1-2kb | 3822 | 3822 |
RSII-PolyA-amplified-1h-Manual Gel 3kb+ | 3110 | 3110 |
RSII-PolyA-amplified-1h-Manual Gel 2-3kb | 483 | 510 |
RSII-PolyA-amplified-4h-Manual Gel 3kb+ | 2645 | 2928 |
RSII-PolyA-amplified-4h-Manual Gel 2-3kb | 14348 | 15708 |
RSII-PolyA-amplified-8h-Manual Gel 3kb+ | 3443 | 3765 |
RSII-PolyA-amplified-8h-Manual Gel 2-3kb | 1608 | 1765 |
RSII-random-amplified-1, 2, 4, 6, 8h | 1804 | 1953 |
RSII-random-amplified-1h | 2327 | 2485 |
RSII-random-amplified-4h | 7202 | 7629 |
RSII-random-amplified-8h | 11452 | 12259 |
RSII-random-amplified-1, 4, 8h,-Manual Gel 3kb+ | 1778 | 1855 |
RSII-random-amplified-1, 4, 8h,-Manual Gel 1-2kb | 2218 | 2425 |
RSII-random-amplified-1, 4, 8h,-Manual Gel 2-3kb | 3393 | 3627 |
RSII-PolyA-non amplified-1h (1st) | 74 | 81 |
RSII-PolyA-non amplified-1h (2nd) | 7392 | 7440 |
RSII-PolyA-non amplified-2h (1st) | 124 | 125 |
RSII-PolyA-non amplified-2h (2nd) | 12944 | 13121 |
RSII-PolyA-non amplified-4h (1st) | 1369 | 1574 |
RSII-PolyA-non amplified-4h (2nd) | 1023 | 1054 |
RSII-PolyA-non amplified-6h (1st) | 2144 | 2269 |
RSII-PolyA-non amplified-6h (2nd) | 10151 | 10231 |
RSII-PolyA-non amplified-8h (1st) | 219 | 237 |
RSII-PolyA-non amplified-8h (2nd) | 7915 | 8015 |
RSII-PolyA-non amplified-12h (1st) | 935 | 1002 |
RSII-PolyA-non amplified-12h (2nd) | 12731 | 12864 |
aType of the samples.
bTotal number of PRV-specific reads.
cTotal number of reads mapped to the PRV genome. (There is an approximately 15 kb-long inverted repeat sequence region in the PRV genome, therefore those reads which map to this location occur in duplicate in row c).