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. 2018 Jun 19;5:180119. doi: 10.1038/sdata.2018.119

Table 3. Summary table of the various wet lab approaches used in this study.

Sample No Sample Sample time points (h pi) RT priming Cap-selection Amplification Size selection Library prep Platform
1 Mixed 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 Oligo(d)T no yes no Illumina single-end HiScan SQ
2 Mixed 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 Random no yes no Illumina paired-end HiScan SQ
3 1 h 1 Oligo(d)T no yes no PacBio Isoform seq RSII
4 4 h 4 Oligo(d)T no yes no PacBio Isoform seq RSII
5 8 h 8 Oligo(d)T no yes no PacBio Isoform seq RSII
6 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes Bluepippin 5kb+ PacBio Isoform seq RSII
7 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes Bluepippin 0.8-2kb PacBio Isoform seq RSII
8 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes Bluepippin 2-3kb PacBio Isoform seq RSII
9 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes Bluepippin 3-5kb PacBio Isoform seq RSII
10 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes Bluepippin 5kb+ PacBio Isoform seq RSII
11 Mixed 1, 4, 8 Oligo(d)T no yes Manual Gel 1-2kb PacBio Isoform seq RSII
12 1 h 1 Oligo(d)T no yes Manual Gel 3kb+ PacBio Isoform seq RSII
13 1 h 1 Oligo(d)T no yes Manual Gel 2-3kb PacBio Isoform seq RSII
14 4 h 4 Oligo(d)T no yes Manual Gel 3kb+ PacBio Isoform seq RSII
15 4 h 4 Oligo(d)T no yes Manual Gel 2-3kb PacBio Isoform seq RSII
16 8 h 8 Oligo(d)T no yes Manual Gel 3kb+ PacBio Isoform seq RSII
17 8 h 8 Oligo(d)T no yes Manual Gel 2-3kb PacBio Isoform seq RSII
18 Mixed 1, 2, 4, 6, 8 Random no yes no PacBio Isoform seq RSII
19 1 h 1 Random no yes no PacBio Isoform seq RSII
20 4 h 4 Random no yes no PacBio Isoform seq RSII
21 8 h 8 Random no yes no PacBio Isoform seq RSII
22 Mixed 1, 4, 8 Random no yes Manual Gel 3kb+ PacBio Isoform seq RSII
23 Mixed 1, 4, 8 Random no yes Manual Gel 1-2kb PacBio Isoform seq RSII
24 Mixed 1, 4, 8 Random no yes Manual Gel 2-3kb PacBio Isoform seq RSII
25 1 h 1 Oligo(d)T no no no PacBio 2kb RSII
26 1 h 1 Oligo(d)T no no no PacBio Very Low Input RSII
27 2 h 2 Oligo(d)T no no no PacBio 2kb RSII
28 2 h 2 Oligo(d)T no no no PacBio Very Low Input RSII
29 4 h 4 Oligo(d)T no no no PacBio 2kb RSII
30 4 h 4 Oligo(d)T no no no PacBio Very Low Input RSII
31 6 h 6 Oligo(d)T no no no PacBio 2kb RSII
32 6 h 6 Oligo(d)T no no no PacBio Very Low Input RSII
33 8 h 8 Oligo(d)T no no no PacBio 2kb RSII
34 8 h 8 Oligo(d)T no no no PacBio Very Low Input RSII
35 12 h 12 Oligo(d)T no no no PacBio 2kb RSII
36 12 h 12 Oligo(d)T no no no PacBio Very Low Input RSII
37 Mixed 1, 2, 4, 6, 8 Oligo(d)T no yes no PacBio Isoform seq Sequel
38 Mixed 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 18, 24 Oligo(d)T no no no ONT Direct RNA MinION
39 Mixed 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 18, 24 Oligo(d)T yes yes no ONT cDNA MinION
40 Mixed 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 18, 24 Oligo(d)T no yes no ONT cDNA MinION