Table 4. Summary table of the reagents and chemistries used for the sequencing.

Total RNA isolation	PolyA selection	Ribodepletion	Reverse transcription & dscDNA production	cDNA synthesis by PCR	Library preparation kit	Sequencing chemistry	Instrument
Macherey-Nagel RNA	Qiagen Oligotex mRNA mini Kit	–	StarScript AMV Reverse Transcriptase	FailSafe PCR PreMix Selection Kit	ScriptSeq v2 RNA-Seq Library Preparation Kit	NA	HiScan 2500
	–	Epicentre Ribo-Zero™ Magnetic Kit H/M/R
	Qiagen Oligotex mRNA mini Kit	–	SuperScript III & SuperScript double-stranded cDNA Synthesis Kit	–	PacBio Template Preparation Kit	P5-C3	RSII
			Clontech SMARTer PCR cDNA Synthesis Kit	KAPA HiFi PCR Kit		P6-C4
	–	Epicentre Ribo-Zero™ Magnetic Kit H/M/R
	Qiagen Oligotex mRNA mini Kit	–					Sequel
			SuperScript III	–	Direct RNA Sequencing Kit	NA	MinION
			SuperScript IV	KAPA HiFi PCR Kit	Ligation Sequencing Kit 1D	NA
			Lexogen Teloprime Kit enzymes & reagents	Lexogen Teloprime PCR mix	Ligation Sequencing Kit 1D	NA