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. 2018 Jun 19;5:180119. doi: 10.1038/sdata.2018.119

Table 5. The list of primers used in this study for the reverse transcription reactions.

Sequencing method Name, availability Catalog # Sequence (5' -> 3')
Illumina PolyA Custom made-adaptor-primer VN(T)20 (IDT DNA) - GTGTGCTCTTCCGATCT(T)20VN
Illumina random Random hexamer + tagging sequence-ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) SSV21106 & SSV21124 GTGTGCTCTTCCGATCTNNNNNN
PacBio non-amplified Anchored Oligo(dT)20 primers (Life Technologies) 12577011 TTTTTTTTTTTTTTTTTTTTVN
PacBio amplified PolyA 3' SMART CDS primer II A-SMARTer PCR cDNA Synthesis Kit (Clontech) 634925 & 634926 AAGCAGTGGTATCAACGCAGAGTAC(T)30VN
PacBio amplified Random Custome made (IDT DNA) - AAGCAGTGGTATCAACGCAGAGTACNNNNNN (G: 37%; C: 37%; A: 13%; T: 13%)
MinION cDNA Poly(T)-containing anchored primer [(VN)T20-ONT recommended, custom made (Bio Basic) - 5phos/ ACTTGCCTGTCGCTCTATCTTC(T)20VN
MinION CAP selected TeloPrime Full-Length cDNA Amplification Kit (Lexogen) 013.08 & 013.24 TCTCAGGCGTTTTTTTTTTTTTTTTTT
MInION RNA RT adapter-Direct RNA Sequencing Kit (Oxford Nanopore Technologies) SQK-RNA001 GAGGCGAGCGGTCAATTTTCCTAAGAGCAAGAAGAAGCCTTTTTTTTTT