FIG 3.

Persistence of L. interrogans measured by qPCR and PMA-qPCR in sterile and nonsterile microcosms. (A and B) Spring water; (C and D) Brazilian soil. Squares denote measurements by qPCR, and circles denote measurements by PMA-qPCR. The solid line represents the modeled curve for qPCR measurements, and the dashed line represents the modeled curve for PMA-qPCR measurements. Open symbols represent data points for which at least one observation was below the limit of detection. Error bars indicate standard deviations. The horizontal dashed line indicates the limit of detection.