TABLE 4.
Primer sequences and PCR conditions
| Primer | Sequence (5′→3′) | Amplicon size (bp) | Annealing temp (°C) | Source or reference |
|---|---|---|---|---|
| sepA-f | TGTACTTTCTGGTGCGAT | 176 | 52 | This study |
| sepA-r | GCTCGACTGCAAATATGA | |||
| mepA-f | TGCTATCTCTCTAACACTGCCA | 657 | 56 | This study |
| mepA-r | GCGAAGTTTCCATAATGTGC | |||
| norA-f | GATTGGTGGATTTATGGCAG | 509 | 56 | This study |
| norA-r | TTGTAATGGCTGGTCGTATC | |||
| mco-f | AAATGGCTCCAATGCTCG | 418 | 50 | This study |
| mco-r | ACGGGTGCTTCATACCACTC | |||
| lmrS-f | TGATGTCAATGGTTGGACC | 462 | 58 | This study |
| lmrS-r | AATGCGATGGCGATGTAG | |||
| smr-f | ATAAGTACTGAAGTTATTGGAAGT | 285 | 50 | 40 |
| smr-r | TTCCGAAAATGTTTAACGAAACTA | |||
| qacA/B-f | ATTCCATTGAGTGCCTTTGC | 198 | 52 | 41 |
| qacA/B-r | TGGCCCTTTCTTTAGGGTTT | |||
| qacH-f | CAAGTTGGGCAGGTTTAGGA | 121 | 52 | 41 |
| qacH-r | TGTGATGATCCGAATGTGTTT | |||
| qacG-fw | CAACAGAAATAATCGGAACT | 275 | 48 | 42 |
| qacG-rv | TACATTTAAGAGCACTACA | |||
| qacJ-fw | CTTATATTTAGTAATAGCG | 306 | 48 | 42 |
| qacJ-rv | GATCCAAAAACGTTAAGA | |||
| czrC-f | TAGCCACGATCATAGTCATG | 655 | 56 | 43 |
| czrC-r | ATCCTTGTTTTCCTTAGTGACTT | |||
| copA-f | CATGCTTTAGGCTTGGCAAT | 662 | 55 | 43 |
| copA-r | TCTTCTGGCATGAGTTGTGC |