FIG 2.

Reconstruction of the central metabolism of C. thermosuccinogenes. Green arrows denote reactions verified in enzyme assays with C. thermosuccinogenes cell extract. 6PGDH, 6-phosphogluconate dehydrogenase; ACDH, acetaldehyde dehydrogenase; ACN, aconitase; ACS, acetyl-CoA synthetase; ADH, alcohol dehydrogenase; AK, acetate kinase; CS, citrate synthase; ENO, enolase; FBA, fructose-bisphosphate aldolase; FH, fumarate hydratase; FR, fumarate reductase; G6PDH, glucose-6-phosphate dehydrogenase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GK, glucokinase; ICD, isocitrate dehydrogenase; LDHL, l-lactate dehydrogenase; MDH, malate dehydrogenase; ME, malic enzyme; OAD, oxaloacetate; decarboxylase; OOR, 2-oxoglutarate,ferredoxin oxidoreductase; PEPCK, phosphoenolpyruvate carboxykinase; PFK, phosphofructokinase; PFL, pyruvate formate lyase; PFOR, pyruvate:ferredoxin oxidoreductase; PGI, phosphoglucose isomerase; PGK, phosphoglycerate kinase; PGLS, 6-phosphogluconolactonase; PPdK, pyruvate, phosphate dikinase; PTA, phosphate acetyltransferase; PYK, pyruvate kinase; RPE, ribulose-phosphate 3-epimerase; RPI, ribose-5-phosphate isomerase; TAL, transaldolase; TKL, transketolase; TPI, triosephosphate isomerase; XI, xylose isomerase; XK, xylulokinase.