TABLE 2.

Enzyme assay with E. coli cell extract containing the two MDH candidates from C. thermosuccinogenes, which were initially annotated as LDHs

Enzyme	Reaction velocity (μmol/mg cell extract protein/min)a
	MDHb assay		LDHc assay
	NADH	NADPH	NADH	NADPH
CDQ83_08825	5.35 ± 0.04	6.27 ± 0.86	NDd	ND
CDQ83_04860	1.34 ± 0.42	ND	0.96 ± 0.13	ND

^aReaction velocities are given ± the standard deviations.

^bMDH, malate dehydrogenase.

^cLDH, lactate dehydrogenase.

^dND, not detected.