TABLE 3.
Enzyme assay with C. thermosuccinogenes cell extract to determine cofactor specificities for several glycolytic reactions
| Reaction | Cofactor | Reaction velocity (μmol/mg cell extract protein/min)a in cell extracts grown on: |
|
|---|---|---|---|
| Glucose | Xylose | ||
| Glucokinase | ATP | NDb | ND |
| GTP | 0.90 ± 0.10 | 0.68 ± 0.12 | |
| PPi | ND | ||
| Xylulokinase | ATP | 0.01 ± 0.01 | 0.60 ± 0.01 |
| GTP | 0.02 ± 0.00 | 0.83 ± 0.05 | |
| Malic enzyme | NADP+ | 2.5 ± 0.3 | 2.3 ± 0.3 |
| NAD+ | ND | ND | |
| 6-Phosphofructokinasec | ATP | ND | |
| GTP | ND | ||
| PPi | 2.8 ± 0.2 | 4.0 ± 0.2 | |
| Phosphoglycerate kinasec | ATP | 2.8 ± 0.3 | 2.9 ± 0.2 |
| GTP | 1.2 ± 0.1 | 1.1 ± 0.1 | |
| PPi | |||
a
Reaction velocities are given ± the standard deviations.
b
ND, not detected.
c
Cell extract prepared with Tris-HCl buffer instead of potassium phosphate buffer.