TABLE 3.
Primers and vectors used in this study
| Enzyme and source | Vector | Primer | DNA sequence (5′–3′)a | Restriction enzyme |
|---|---|---|---|---|
| B. fragilis NCTC9343 | ||||
| BfGH33A | pET-21b | BfGH33AF | CAGCCATATGCGTGAAACACGTATTCCTAT | NdeI |
| BfGH33AR | CGCGCTCGAGTTTAATAATGTCTTTCAACTTC | XhoI | ||
| BfGH33B | pET-21b | BfGH33BF | CGAGCATATGAAAGTTATTGTTAGACAGCCCA | NdeI |
| BfGH33BR | CGAGCTCGAGTCTCTCTTTATATGATTTACCTTT | XhoI | ||
| BfGH33C | pET-21b | BfGH33CF | CGAGGGATCCGGATACTATTTTTGTTTATGAA | BamHI |
| BfGH33CR | CGATCTCGAGTTGTATTATATCTCTCAGTCGGA | XhoI | ||
| C. perfringens ATCC 13124 | ||||
| CpGH33A | pET-21b | CpGH33AF | CGCGCATATGAAAAGTAAAAAAATTATAGC | NdeI |
| CpGH33AR | CCGCTCGAGCCTAGCAGTTCTTATAGTCTT | XhoI | ||
| CpGH33B | pET-21b | CpGH33BF | CGCGCTAGCATGAATTACAAAGGGATAAC | NheI |
| CpGH33BR | CGCGCTCGAGTTTATTAGCTCCACTCTCT | XhoI | ||
| CpGH33C | pET-28b | CpGH33CF | CGCGCTAGCATGTATAACAAAAACAACACCTT | NheI |
| CpGH33CR | CGCGCTCGAGTTGTTTATTAATTAGTGAGT | XhoI | ||
| B. bifidum JCM1254 | ||||
| SiaBb2 | pET-21b | SiaBb2F | CGACATATGGCCAGCGATGATGCTGACATG | NdeI |
| SiaBb2R | CGACTCGAGCTTGGACAGGCCGGGCTGCTC | XhoI | ||
a
Restriction enzyme sites are underlined.