FIG 3.

Natural-abundance ¹³C NMR spectra of ethanolic extracts from E. coli BL21(DE3) cells harboring different recombinant plasmids grown in LB medium after induction with IPTG. Details for the induction of protein with IPTG and sample preparation are given in Materials and Methods. (A) E. coli BL21(DE3) harboring pACYC-bmoA. (B) E. coli BL21(DE3) harboring pACYC-bmoB. (C) E. coli BL21(DE3) harboring pACYC-bmoA-bmoB. (D) E. coli BL21(DE3) harboring pACYCDuet-1. B, glycine betaine; D, dimethylglycine.