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Effects of the molar ratios of BmoA to BmoB on BMO activity. The specific activities were measured by the amount of formaldehyde produced after shaking at 30°C for 30 min while keeping the concentration of BmoB constant at 1.5 μM and varying the concentration of BmoA (1.5 to 75 μM) in the reaction mixture containing 10 μM FAD, 5 mM NADH, and 2 mM GB.
