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. 2018 Jun 18;84(13):e00377-18. doi: 10.1128/AEM.00377-18

FIG 8.

FIG 8

Identification of the putative Rieske-type protein involved in GB-to-dimethylglycine degradation from C. salexigens DSM 3043. (A) Organization of the cofactor binding domains in class IA, class IB, and BmoB. (B) Classification of the large subunits of 39 terminal oxygenase components in Rieske nonheme iron oxygenases. The phylogenetic tree, inferred by the neighbor-joining method, shows the relative positions of BmoA among other characterized ROs based on the Nam classification system (42). Bootstrap values for 1,000 replications are given. The scale bar represents two substitutions per 10 amino acids. The protein abbreviations, their corresponding species, and gi/accession numbers in the GenBank database are as follows: OxoO, P. putida 86, 2072732; CarAa, Pseudomonas sp. strain CA10, 2317678; VanA, Pseudomonas sp. HR199, 1946284; DdmC, Pseudomonas maltophilia DI-6, 55584974; TsaM, Comamonas testosteroni T-2, 1790867; PobA, P. pseudoalcaligenes POB310, 473250; LigX, Sphingomonas paucimobilis SYK-6, 4062861; NdmA, P. putida CBB5, AFD03116; NdmB, P. putida CBB5, AFD03117; BphA1, P. pseudoalcaligenes KF707, 151091; IpBAa, P. putida RE204, 2822265; BpdC1, Rhodococcus sp. strain M5, 927232; BedC1, P. putida ML2, 1168640; HcaE, E. coli K-12, 81170783; BphA1, Rhodococcus erythropolis TA421, 3059209; PhnA1a, Sphingomonas sp. strain CHY-1, 75455648; ArhA1, Sphingomonas sp. strain A4, 50725019; NahAc, Pseudomonas sp. strain JS42, 1224114; DntAc, Burkholderia cepacia R34, 17942397; PhtAa, Mycobacterium vanbaalenii PYR-1, 49072886; NarAa, Rhodococcus sp. strain NCIMB12038, 4704462; NidA, M. vanbaalenii PYR-1, 11038552; PhdA, Nocardioides sp. strain KP7, 7619816; NidA3, M. vanbaalenii PYR-1, 68053509; CntAb, P. putida F1, 1263180; PsbAb, Rhodopseudomonas palustris, 5360700; AtdA, Acinetobacter sp. strain YAA, 1395141; TdnA1, P. putida UCC22, 1841362; AntA, Acinetobacter sp. ADP1, 3511232; CbdC, Burkholderia cepacia 2CBS, 758210; XylX, P. putida TOL, 139861; CmoS, Spinacia oleracea, AAB52509; CmoB, Beta vulgaris, AAB80954; CmoA, Amaranthus tricolor, AAK82768; CntA, A. baumannii ATCC 19606, EEX03955; OxyBAC, Pseudomonas sp. BIOMIG1, 1057213843; Stc2, S. meliloti 1021, 16262853; BmoA, C. salexigens DSM 3043, 759867680; and GbcA, P. aeruginosa PAO1, 15600603. (C) Multiple-sequence alignment of BmoA and the members in group V of the RO family. The asterisk (*) indicates the conserved glutamate residue in the mononuclear iron center domain of microbial RO oxygenase component, while identical amino acids residues in the eight sequences are shown in black.