TABLE 1.
Apparent kinetic parameters for the reductase activity of His6-BmoBa
| Substrate (concn range [μM] | Vmax (μmol · mg−1 · min−1) | Km (μM) | kcat (s−1) | Cosubstrate |
|---|---|---|---|---|
| NADH (12.5–200)b | 1.25 ± 0.07 | 43.94 ± 1.4 | 0.90 ± 0.01 | FAD |
| NADPH (12.5–800)b | 0.49 ± 0.05 | 257.80 ± 2.6 | 0.35 ± 0.01 | FAD |
| FAD (2.5–200)c | 5.90 ± 0.18 | 6.03 ± 0.13 | 4.25 ± 0.01 | NADH |
| FMN (2.5–200)c | 6.79 ± 0.12 | 17.31 ± 0.11 | 4.89 ± 0.01 | NADH |
| Riboflavin (2.5–500)c | 2.32 ± 0.15 | 111.83 ± 6.75 | 1.67 ± 0.01 | NADH |
a
Experiments were performed in 50 mM Tris-HCl (pH 7.0) at 25°C. Values are means of and standard deviations of the results from three independent sets of experiments.
b
Determined with a fixed FAD concentration of 150 μM.
c
Determined with a fixed NADH concentration of 200 μM.