TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| Chromohalobacter salexigens | ||
| DSM 3043 | Wild type | DSMZb |
| ZW4-1 | Spontaneous rifampin-resistant mutant of C. salexigens DSM 3043 | 23 |
| 3043ΔbmoA | bmoA in-frame deletion mutant of strain ZW4-1, Rifr | This study |
| 3043ΔbmoB | bdmB in-frame deletion mutant of strain ZW4-1, Rifr | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (Φ801acZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | TaKaRa |
| S17-1 | pro hsdR recA [(RP4-2 (Tc::Mu)(Km::Tn7))] | 23 |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3); T7 RNA polymerase gene under the control of the lacUV promoter | TianGen |
| Plasmids | ||
| pK18mobsacB | mob sacB (RP4) lacZ, Kmr | 54 |
| pK18mobsacB::ΔbmoA | pK18mobsacB carrying truncated bmoA (csal_1004) gene | This study |
| pK18mobsacB::ΔbmoB | pK18mobsacB carrying truncated bmoB (csal_1005) gene | This study |
| pBBR1MCS | Broad-host-range vector, Cmr | 55 |
| pBBR1MCS-bmoA | pBBR1MCS-2 carrying bmoA and its predicted native promoter region, Cmr | This study |
| pBBR1MCS-bmoB | pBBR1MCS carrying bmoB and its predicted native promoter region, Cmr | This study |
| pBBR1MCS-bmoA-bmoB | pBBR1MCS carrying bmoA and bmoB and their predicted native promoter regions, Cmr | This study |
| pACYCDuet1 | Dual protein expression vector, Cmr | Novagen |
| pACYC-bmoA | pACYCDuet1 carrying bmoA in MCS-1 under the control of the T7 promoter, Cmr | This study |
| pACYC-bmoB | pACYCDuet1 carrying bmoB in MCS-2 under the control of the T7 promoter, Cmr | This study |
| pACYC-bmoA-bmoB | pACYCDuet1 carrying bmoA in MCS-1 and bmoB in MCS-2 under the control of their respective T7 promoters, Cmr | This study |
| pET-28a(+) | Expression vector, Kmr | Novagen |
| pET28a-bmoA | pET28a carrying bmoA under the control of the T7 promoter (BmoA-His6) | This study |
| pET28a-bmoB | pET28a carrying bmoB under the control of the T7 promoter (His6-BmoB) | This study |
a
Cmr, chloramphenicol resistant; Kmr, kanamycin resistant.
b
DSMZ, German Collection of Microorganisms and Cell Cultures.