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Overview of the RNA-seq outcome, including numbers for total raw, high-quality reads and nucleotides and statistical Q20, Q30, and GC percentagesa
| Transcriptome | No. of raw reads | No. of clean reads | No. of clean bases | Q20 (%) | Q30 (%) | GC (%) |
|---|---|---|---|---|---|---|
| Sample A | 20,106,548 | 19,791,168 | 2.47 billion | 96.18 | 92.03 | 51.82 |
| Sample B | 18,486,306 | 18,139,002 | 2.27 billion | 96.02 | 91.92 | 50.86 |
Q20 and Q30 represent the percentages of nucleotide bases with Phred quality scores (Q) of 20 and 30, respectively.
