FIG 8.

Intracellular Ca²⁺ regulates hyphal branching and GA biosynthesis by regulating the ROS level under copper stress. (A) Changes in the ROS levels in G. lucidum were determined using DCFH-DA staining after treatment with a Ca²⁺-related inhibitor, CaCl₂, or 3 mM copper. Scale bar = 100 μm. (B) Changes in the ROS fluorescence ratio in the hyphal regions of G. lucidum after treatment with a Ca²⁺-related inhibitor, CaCl₂, or 3 mM copper. The y axis represents the ROS fluorescence ratio measured by CLSM, and the x axis represents the different treatments. The values are the mean ± SD of the results from three independent experiments. Within each set of experiments, different letters indicate significant differences between the lines (P < 0.05, according to a multiple-comparison one-way ANOVA).