FIG 6.

Proposed catalytic mechanism for PolG. (A) Proposed mechanism for the PolG-catalyzed reaction and scheme for the chromogenic reaction of the hydroxamate complex. Compound 1 is converted to compound 2, which might react with Fe³⁺ to form the hydroxamate-Fe³⁺ complex (dark brown). (B) Detection of compound 2 by chromogenic reaction. I, Fe³⁺ solution only; II, negative control of the PolG reaction with NH₂OH·HCl and Fe³⁺ added; III, PolG reaction with Fe³⁺ added; IV, PolG reaction with NH₂OH·HCl and Fe³⁺ added (dark brown). (C) LC-MS analysis of the PolG reaction with NH₂OH.