Figure 2.

The sticky end sequences, sticky end energies, and the stability in the presence of T7 RNAP in transcription conditions of nanotubes with six different 7 bp sticky end sequences. The domains of variants var2_7 to var6_7 whose sequences are different from the sequences of the respective domains in var1_7 are shown in different colors than the domains in var1_7 (SI Section 1 for sequences). Free energies were estimated for comparison using NUPACK (72) (SI Section 8.1). Nanotubes were deemed stable (check) at the enzyme unit concentration listed if nanotubes at 1 μM tile concentration were present after 24 h of incubation with T7 RNAP in transcription conditions and were deemed unstable (X) if no nanotubes were present after this incubation. Fluorescence micrographs of the nanotubes after 24 h of incubation with and without T7 RNAP are shown in Supplementary Figure S7.