Figure 5.

RNA transcripts produced when var1_7-3 DNA nanotubes are incubated with T7 RNAP in transcription conditions bind to the single-stranded overhang domain of the DNA tiles during nanotube disassembly. (A) Schematic showing the process of RNA hybridization and fluorescence increase. RNA transcripts bind to the single-stranded overhang and hybridization of the RNA and overhang increases the fluorescence of the FAM molecule at the 5′ end of the overhang. This hybridization can also disassemble tiles, inactivating them for nanotube assembly and/or causing them to detach from nanotubes. (B) Normalized FAM fluorescence of nanotubes in transcription conditions with a full set of NTPs or just ATP before and after the addition T7 RNAP. Fluorescence values for each sample are normalized to the fluorescence of the sample at t = 0. T7 RNAP was added at the time indicated by the red arrow. (C) Fluorescence micrographs of the samples in B after different incubation times. Scale bars: 10 μm.