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. 2018 Mar 15;46(10):4950–4965. doi: 10.1093/nar/gky196
Length Amplicon length Melting temp. gc% seq
Pcdhb5 LEFT PRIMER 20 86 60.69 50 TGCTTCCTGGTCCTTTGTGT
RIGHT PRIMER 20 86 60.93 50 CCCATTCAAGGTCGGGTATT
Pcdhb14 LEFT PRIMER 20 100 60.1 50 GCAAGTGACCGCCATTATCT
RIGHT PRIMER 19 100 60.24 57.89 CACCAAAGAGCCCCTGTCT
Pcdhb15 LEFT PRIMER 19 108 59.78 63.16 GGCACCCCTCTCTTCTCAC
RIGHT PRIMER 21 108 59.66 42.86 CCCCAAATTCCTTCCATTTAG
Pcdhb22 LEFT PRIMER 21 80 60.01 47.62 GGTGCAGAAGCAAAGATGAAG
RIGHT PRIMER 20 80 59.84 50 ACCACTCCCAGCAAGAGAAA