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. 2018 Jun 4;4(1):vey012. doi: 10.1093/ve/vey012

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Declining virus titers during Aag2 passage. (A) Declining virus titers for eight populations passaged on Aag2 cells. The right panel shows measured titers from plaque assays on BHK cells 48 hpi. The left panel shows estimated inoculum size for each passage. (B) Estimates of specific infectivity for the ancestral virus stock used to initiate experimental passage and three populations (P1, P2, and P3) after six passages on Aag2 cells. Copy number was estimated by quantitative PCR, and titer is from plaque assays on BHK cells.