Table 1.
PCR primers used for WNV amplification.
| Amplicon | Positiona (forward–reverse) | Sequence (5'-3') | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| 1 | 2–2,493 | GTAGTTCGCCTGTGTGAGCT | 60.0 | 2492 |
| GATGTCTATGGCACACCCAGT | 59.9 | |||
| 2 | 2,336–4,647 | TGTCCTGGATAACGCAAGGAT | 59.2 | 2312 |
| CTCCTTTGGTGAGGGAGTGTC | 60.0 | |||
| 3 | 4,488–6,793 | TCCAGGAGCACCTTGGAAGA | 60.5 | 2306 |
| GCAACATTCCGGCGATCTTCG | 62.6 | |||
| 4 | 6,603–8,907 | GGAACTGCCAGATGCTCTTC | 60.0 | 2305 |
| TGCATTGCTGTTGACCTTTC | 59.9 | |||
| 5 | 8,755–1,1027 | GAGAAGGTGGACACGAAAGC | 58.9 | 2273 |
| ATCCTGTGTTCTCGCACCAC | 61.2 |
a
Position on the genome sequence is based on GenBank accession no. JF957173.