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. 2018 Mar 6;46(10):5209–5226. doi: 10.1093/nar/gky159

Figure 5.

Figure 5.

Mitochondrial tRNA polyadenylation and turnover are influenced by drugs that inhibit ATP synthesis or protein synthesis. Northern blots probed for mitochondrial tRNASer(UCN), in 143B wild-type cybrid cells treated with or without EtBr for the indicated times: (A) plus the indicated concentrations of oligomycin, with one additional hour of pre-treatment; (B) plus the indicated concentrations of CCCP, with one additional hour of CCCP pre-treatment, or CCCP addition one hour later, as shown. Final two tracks were from cells treated only with CCCP for 6 h. See also Supplementary Figure S5. In other panels cells pre-treated for 1 h with the indicated doses of (C) doxycycline (DOX), (D) thiamphenicol (TAP) or (E) puromycin (PURO), and then for a further 6 h with (+) or without (–) concomitant treatment with 5 μg/ml EtBr, as shown. (F) Treatment with (+) or without (–) 200 μg/ml doxycycline, plus 5 μg/ml EtBr added and withdrawn at times indicated by arrows. Asterisk denotes tRNA modified at the highest dose of doxycycline, in the absence of EtBr.