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. 2018 Mar 6;46(10):5209–5226. doi: 10.1093/nar/gky159

Figure 7.

Figure 7.

Polyadenylation of mutant tRNALeu(UUR) is induced by doxycycline, not EtBr. Northern blots of mitochondrial tRNAs, denoted by their decoding specificities, in A549 or GT cybrid cells as indicated: (A) cells treated with the indicated doses of doxycycline (DOX) with (+) or without (–) concomitant treatment with 5 μg/ml EtBr, for the times shown (plus 1 h of preincubation with DOX where applicable), or (B) in cells treated for the indicated times with 5 μg/ml EtBr. Control treatment with an equivalent volume of 70% ethanol, the solvent used for delivery of doxycycline, denoted by e. Note that control A549 cells are wild-type for tRNALeu(UUR), whereas GT cells are homoplasmic for the A3243G mutant tRNALeu(UUR). See Figure S6A for longer exposure of the tRNALeu(UUR) blot from GT cells. Note that the steady-state level of tRNALeu(UUR) in GT cells compared with control cells is low, due to the A3243G mutation, as seen in part (B). Thus, a much longer exposure is shown for the tRNALeu(UUR) panel than for the tRNASer(UCN) panel in part (A).