Figure 9.

nPKCε and cPKCβI modulate Munc18-1 phosphorylation under muscle contraction conditions. (A) Representative Western blot bands and quantification show that Munc18-1 phosphorylation and the pMunc18-1/Munc18-1 ratio are significantly decrease after stimulation resulting in contraction in nPKCε inhibitor peptide εV1-2 preincubated muscles. (B) Representative Western blot bands and quantification show that the pMunc18-1/Munc18-1 ratio are significantly decreases after stimulation resulting in contraction in cPKCβI inhibitor peptide βIV5-3 preincubated muscles. (C) Representative Western blot bands and quantification show that the phosphorylation of nPKCε and the pnPKCε/nPKCε ratio significantly increases after stimulation resulting in contraction in cPKCβI inhibitor peptide βIV5-3 preincubated muscles. Data are mean percentage ± SEM, ^*p < 0.05 (n = 5).