Figure 4.

Multiple turnover kinetics experiments for unmodified and sumoylated TDG (50 nM) acting on G·fC or G·caC substrates (1000 nM), performed at 37°C. (A) G·fC; linear fitting of data in the steady-state region gives velocities of v₀ = 0.236 ± 0.011 nM·min⁻¹ for TDG, v₀ = 0.340 ± 0.036 nM·min⁻¹ for TDG∼SUMO-1, and v₀ = 0.315 ± 0.022 nM·min⁻¹ for TDG∼SUMO-2. (B) G·caC; linear fitting of data in the steady-state region gives velocities of v₀ = 0.363 ± 0.023 nM·min⁻¹ for TDG, v₀ = 0.356 ± 0.040 nM·min⁻¹ for TDG∼SUMO-1, and v₀ = 0.354 ± 0.037 nM·min⁻¹ for TDG∼SUMO-2.