Table 2. Steady-state catalytic activity for unmodified and sumoylated TDG.
Enzyme | Substrate | v o (nM·min−1) | k cat (min−1) | k max/kcat |
---|---|---|---|---|
TDG | G·fC | 0.236 ± 0.011 | (4.7 ± 0.3) × 10−3 | 359 |
TDG∼SUMO-1 | G·fC | 0.340 ± 0.036 | (6.8 ± 0.7) × 10−3 | 12 |
TDG∼SUMO-2 | G·fC | 0.315 ± 0.022 | (6.3 ± 0.5) × 10−3 | 12 |
TDG | G·caC | 0.363 ± 0.023 | (7.3 ± 0.5) × 10−3 | 68 |
TDG∼SUMO-1 | G·caC | 0.356 ± 0.040 | (7.1 ± 0.8) × 10−3 | 10 |
TDG∼SUMO-2 | G·caC | 0.354 ± 0.037 | (7.1 ± 0.8) × 10−3 | 10 |