Figure 8.

Associations of the uORFs and dORFs with the main protein coding ORF. (A–F) All the annotated canonical protein coding ORFs were sorted based on the fold change of their normalized RPF read counts upon heat shock (E) or oxidative stress (F) versus the normal condition. On this background, the ORFs with upstream uORFs (A, B) or downstream dORFs (C, D) in the same mRNA transcripts were marked as vertical bars. The color of these bars represents the fold change of the RPF read counts on the uORF (A, B) or dORF (C, D). (G–J) Four examples of the uORF (G, I) or dORF (H, J) that appear negatively associated with the main ORF in response to oxidative stress (G, H) or heat shock (I, J). The colored bar on each nucleotide position represents the count of RPF reads allocated according to its P-site position. Three different colors represent the three frames. The total RPF read counts of the main protein coding ORFs and the uORFs or dORFs are given in the figures.