Table 3.
Statistical analyses (F- and P-values) of the effects of local hormonal application on gene expression levels in Brassica rapa roots. The expression levels of VSP2, PR1, ERF1 and BrLEA4 were measured in B. rapa roots after MeJA, ABA, SA or ethephon application to the roots (n = 3–4 per treatment and harvest time). The data were analysed per hormonal treatment group using a two-way ANOVA model containing treatment (control, hormonal application), time (4, 8, 24, 48 h) and their interaction term as factors. Statistically significant effects (P ≤ 0.05) are indicated in bold.
| Treatment | Factor | Gene | |||
|---|---|---|---|---|---|
| VSP2 | PR1 | ERF1 | BrLEA4 | ||
| MeJA | Treatment (1) | F 1, 22 = 22.57, P < 0.001 | F 1, 22 = 0.00, P = 0.964 | F 1, 19 = 12.84, P = 0.002 | F 1, 23 = 0.88, P = 0.358 |
| Time (2) | F 3, 22 = 2.19, P = 0.117 | F 3, 22 = 0.57, P = 0.639 | F 3, 19 = 12.16, P < 0.001 | F 3, 23 = 4.61, P = 0.011 | |
| Interaction (1.2) | F 3, 22 = 6.07, P = 0.004 | F 3, 22 = 0.36, P = 0.786 | F 3, 19 = 4.34, P = 0.017 | F 3, 23 = 3.24, P = 0.04 | |
| ABA | Treatment (1) | F 1, 19 = 3.46, P = 0.078 | F 1, 22 = 0.13, P = 0.722 | F 1, 20 = 6.72, P = 0.017 | F 1, 22 = 2.5, P = 0.128 |
| Time (2) | F 3, 19 = 8.2, P = 0.001 | F 3, 22 = 0.50, P = 0.684 | F 3, 20 = 3, P = 0.06 | F 3, 22 = 4.03, P = 0.02 | |
| Interaction (1.2) | F 3, 19 = 0.59, P = 0.626 | F 3, 22 = 1.23, P = 0.322 | F 3, 20 = 0.33, P = 0.805 | F 3, 22 = 6.02, P = 0.004 | |
| SA | Treatment (1) | F 1, 20 = 25.4, P < 0.001 | F 1, 20 = 45.69, P < 0.001 | F 1, 18 = 0.02, P = 0.888 | F 1, 23 = 21.68, P < 0.001 |
| Time (2) | F 3, 20 = 5.51, P = 0.006 | F 3, 20 = 1.31, P = 0.299 | F 3, 18 = 8.25, P = 0.001 | F 3, 23 = 8.75, P < 0.001 | |
| Interaction (1.2) | F 3, 20 = 0.75, P = 0.538 | F 3, 20 = 3.56, P = 0.033 | F 3, 18 = 0.94, P = 0.444 | F 3, 23 = 9.5, P < 0.001 | |
| Ethephon | Treatment (1) | F 1, 18 = 9.62, P = 0.006 | F 1, 22 = 14.97, P = 0.001 | F 1, 20 = 234.81, P < 0.001 | F 1, 24 = 43.49, P < 0.001 |
| Time (2) | F 3, 18 = 0.97, P = 0.43 | F 3, 22 = 0.63, P = 0.606 | F 3, 20 = 5.54, P = 0.006 | F 3, 24 = 4.45, P = 0.013 | |
| Interaction (1.2) | F 3, 18 = 1.65, P = 0.213 | F 3, 22 = 1.15, P = 0.35 | F 3, 20 = 0.72, P = 0.549 | F 3, 24 = 0.81, P = 0.503 | |