Table 4.
Effects of local hormonal application on phytohormone levels in Brassica rapa shoots. The levels of JA, SA and ABA were measured at 4, 8, 24 and 48 h after MeJA, ABA, SA and ethephon application. For each measured phytohormone, the data were analysed per treatment using a two-way ANOVA model containing treatment (control, hormonal application), time (4, 8, 24, 48 h) and their interaction term as factors (n = 3–4 per treatment and harvest time). Statistically significant effects (P ≤ 0.05) are indicated in bold.
| Treatment | Factor | Measured phytohormone | ||
|---|---|---|---|---|
| JA | SA | ABA | ||
| MeJA | Treatment (1) | F 1, 19 = 485.13, P < 0.001 | F 1, 22 = 1.96, P = 0.175 | F 1, 21 = 22.82, P < 0.001 |
| Time (2) | F 3, 19 = 68.46, P < 0.001 | F 3, 22 = 17.41, P < 0.001 | F 3, 21 = 7.67, P = 0.001 | |
| Interaction (1.2) | F 3, 19 = 35.80, P < 0.001 | F 3, 22 = 4.00, P = 0.02 | F 3, 21 = 1.22, P = 0.327 | |
| ABA | Treatment (1) | F 1, 22 = 0.57, P = 0.458 | F 1, 22 = 1.93, P = 0.179 | F 1, 20 = 619.21, P < 0.001 |
| Time (2) | F 3, 22 = 2.69, P = 0.071 | F 3, 22 = 7.87, P = 0.001 | F 3, 20 = 80.47, P < 0.001 | |
| Interaction (1.2) | F 3, 22 = 0.19, P = 0.902 | F 3, 22 = 2.58, P = 0.079 | F 3, 20 = 75.15, P < 0.001 | |
| SA | Treatment (1) | F 1, 22 = 5.81, P = 0.025 | F 1, 19 = 912.11, P < 0.001 | F 1, 21 = 15.09, P = 0.001 |
| Time (2) | F 3, 22 = 2.22, P = 0.114 | F 3, 19 = 39.42, P < 0.001 | F 3, 21 = 3.7, P = 0.028 | |
| Interaction (1.2) | F 3, 22 = 0.29, P = 0.834 | F 3, 19 = 13.89, P < 0.001 | F 3, 21 = 0.6, P = 0.62 | |
| Ethephon | Treatment (1) | F 1, 24 = 2.23, P = 0.148 | F 1, 24 = 0.03, P = 0.873 | F 1, 24 = 10.93, P = 0.003 |
| Time (2) | F 3, 24 = 1.22, P = 0.323 | F 3, 24 = 1.14, P = 0.353 | F 3, 24 = 2.18, P = 0.117 | |
| Interaction (1.2) | F 3, 24 = 0.34, P = 0.794 | F 3, 24 = 0.34, P = 0.798 | F 3, 24 = 1.94, P = 0.149 | |