Table 5.
Effects of local hormonal application on phytohormone levels in Brassica rapa roots. The levels of JA, SA and ABA were measured at 4, 8, 24 and 48 h after MeJA, ABA, SA and ethephon application. For each measured phytohormone, the data were analysed per treatment using a two-way ANOVA model containing treatment (control, hormonal application), time (4, 8, 24, 48 h) and their interaction term as factors (n = 3–4 per treatment and harvest time, except for ABA treatment at 24 h where n = 2). Statistically significant effects (P ≤ 0.05) are indicated in bold.
| Treatment | Factor | Measured phytohormone | ||
|---|---|---|---|---|
| JA | SA | ABA | ||
| MeJA | Treatment (1) | F 1, 22 = 1240.19, P < 0.001 | F 1, 22 = 0.33, P = 0.573 | F 1, 18 = 7.07, P = 0.016 |
| Time (2) | F 3, 22 = 8.59, P < 0.001 | F 3, 22 = 3.07, P = 0.049 | F 3, 18 = 16.12, P < 0.001 | |
| Interaction (1.2) | F 3, 22 = 14.17, P < 0.001 | F 3, 22 = 4.05, P = 0.02 | F 3, 18 = 9.83, P < 0.001 | |
| ABA | Treatment (1) | F 1, 21 = 2.34, P = 0.14 | F 1, 21 = 0.18, P = 0.679 | F 1, 19 = 98.24, P < 0.001 |
| Time (2) | F 3, 21 = 1.69, P = 0.199 | F 3, 21 = 2.98, P = 0.055 | F 3, 19 = 7.69, P = 0.001 | |
| Interaction (1.2) | F 3, 21 = 2.77, P = 0.067 | F 3, 21 = 3.11, P = 0.048 | F 3, 19 = 20.21, P < 0.001 | |
| SA | Treatment (1) | F 1, 23 = 0.89, P = 0.355 | F 1, 23 = 677.76, P < 0.001 | F 1, 21 = 2.22, P = 0.151 |
| Time (2) | F 3, 23 = 3.48, P = 0.032 | F 3, 23 = 9.88, P < 0.001 | F 3, 21 = 2.76, P = 0.068 | |
| Interaction (1.2) | F 3, 23 = 0.67, P = 0.581 | F 3, 23 = 4.90, P = 0.009 | F 3, 21 = 4.44, P = 0.015 | |
| Ethephon | Treatment (1) | F 1, 23 = 2.08, P = 0.163 | F 1, 23 = 0.07, P = 0.793 | F 1, 21 = 10.21, P = 0.004 |
| Time (2) | F 3, 23 = 1.77, P = 0.18 | F 3, 23 = 3.08, P = 0.048 | F 3, 21 = 3.02, P = 0.053 | |
| Interaction (1.2) | F 3, 23 = 0.47, P = 0.71 | F 3, 23 = 2.64, P = 0.074 | F 3, 21 = 0.14, P = 0.935 | |