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. 2018 Jun 5;9(43):27293–27304. doi: 10.18632/oncotarget.25551

Figure 1. FZD5 and RYK mediate the anti-proliferative and pro-apoptotic effects of WNT5A in PC3 cells.

Figure 1

Cells were either single treated using siRNAs (siFZD5, siFZD8, siRYK) or WNT5A plasmid-DNA (pcDNA3.1WNT5A), or treated with a combination of first siRNAs (24 h) and afterwards WNT5A plasmid-DNA (24 h). (A-C) Proliferation of control (white bars) and treated (black) PC3 cells. Proliferation was measured using a BrdU proliferation assay after 48 h. (D-F) Caspase 3/7 activation of control (white bars) and treated (black) PC3 cells. Apoptosis was measured after 48 h with a Caspase Glo® 3/7 assay. Data are shown as mean ± SD. *p<0.05; **p<0.01, ***p<0.001; #p<0.05; ##p<0.01. * vs. control, # vs. WNT5A overexpression.