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. 2018 Jun 5;9(43):27242–27255. doi: 10.18632/oncotarget.25531

Figure 1. Establishment of ALK-TKI–resistant H2228 cells.

Figure 1

(A) H2228-CRR, H2228-ALR and H2228-CER cells are resistant to crizotinib, alectinib and ceritinib, respectively. The results of cell viability assays are shown. Data, mean ± SEM from three independent experiments. (B) FISH analysis shows a decrease of the ALK fusion gene in ALK-TKI–resistant H2228 cells compared with H2228 cells (red, ALK3’; green, ALK5’). The percentage of ALK-FISH positive cells are shown in the lower right corners. (C) Protein levels of p-ALK, ALK, p-EGFR, EGFR, p-AKT, AKT, p-ERK and ERK were analyzed by western blotting. ALK-TKI–resistant cells showed markedly decreased p-ALK and ALK expressions.