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. 2018 Jun 5;9(43):27242–27255. doi: 10.18632/oncotarget.25531

Figure 3. EMT changes and resistance to ALK-TKIs in H2228 cells exposed to TGF-β1.

Figure 3

(A) Morphologic changes showing mesenchymal features in H2228 cells were observed after exposure of cells to 5 ng/mL TGF-β1. (B) Protein expression levels of ALK, AXL and EMT markers, after exposure of 5 ng/mL TGF-β1 for 6 hours to H2228 cells, were examined by western blotting. The expression of p-AXL, AXL, vimentin and ZEB1 increased in a time-dependent manner. (C) Resistance to crizotinib, alectinib and ceritinib was caused by exposure of H2228 cells to 5 ng/mL TGF-β1. The results of cell viability assays are shown.